Revista Chapingo Serie Ciencias Forestales y del Ambiente
Universidad Autónoma Chapingo
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Volume XIX, issue 3, - 2013

MORPHOGENIC RESPONSES IN THE in vitro PROPAGATION OF PECAN (Carya illinoinensis [Wangenh] K. Koch)

Jazmín A. Ávila-Treviño; Jesús Guadalupe Arreola-Ávila; José Luis Rodríguez-de la O; Ricardo Trejo-Calzada; Ricardo David Valdez-Cepeda; Amparo Borja-de la Rosa

Received: 15-09-2013

Accepted: 28-11-2013

Available online: / pages.469-481


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  • descriptionAbstract

    Embryogenic and organogenic responses in pecan (Carya illinoinensis [Wangenh] K. Koch) were observed as a result of the in vitro cultivation of segments of leaves, axillary buds and zygotic embryos. Necrosis was controlled through the use of activated carbon (AC: 1%), polyvinylpyrrolidone (0.1 %), silver nitrate (AgNO3: 1 %), citric acid (150 mg·L-1) and ascorbic acid (100 mg·L-1, in both light and darkness. Murashige and Skoog base medium (MS) was used, supplemented with 0.40 mg·L-1 of thiamine, 100 mg·L-1 of myo-inositol, 3 % saccharose, incorporating 2,4-D for leaves, thidiazuron (TDZ) for embryos, and combinations of benzyladenine (BA), kinetin (KIN) naphthalenacetic acid (ANA) and indolebutyric acid (AIB) for axillary buds. Tissue necrosis was reduced by 75 % and 83 % adding CA and AgNO3, respectively. 33 % and 66 % of embryogenic callus originated from leaves, using 1 and 3 mg·L-1 of 2,4-D. The highest callus production (58 %) from embryos was obtained from the concentration of 3 mg·L-1 of TDZ. In axillary buds, the combination of KIN (3.0 μM), BA (1.0 μM) and AIB (0.3 μM) increased the number of leaves and seedlings, as well as shoot length.

    Keyworks: Explants, callogenesis, antioxidants, growth regulators.
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  • starCite article

    Ávila-Treviño, J. A.,  Arreola-Ávila, J. G.,  Rodríguez-de la O, J. L.,  Trejo-Calzada, R., Valdez-Cepeda, R. D.,  &  Borja-de la Rosa, A. (2013).  MORPHOGENIC RESPONSES IN THE in vitro PROPAGATION OF PECAN (Carya illinoinensis [Wangenh] K. Koch). , XIX(3), 469-481.