Revista Chapingo Serie Ciencias Forestales y del Ambiente
Universidad Autónoma Chapingo
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Revista Chapingo Serie Ciencias Forestales y del Ambiente
Volume XX, issue 2, May - August 2014


Olivia Márquez-Fernández; Luis Á. Juárez Pacheco; Ángel Trigos

Received: 01-12-2013

Accepted: 28-05-2014

Available online: / pages.227-235


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  • descriptionAbstract

    Five compounds in a commercial strain of the mushroom Pleurotus sp. were obtained from ethyl acetate and methanol extracts. The compounds were isolated using techniques such as column and thin layer chromatography. The following sterols were identified by 1H NMR spectroscopy: 1) ergosta-5,7,22-trien-3β-ol (ergosterol), 2) 5α, 8α-epidioxy-22E ergosta-6, 22-dien-3β-ol (ergosterol peroxide), 3) 3β,5α,6β,9α-tetrahidroxiergosta-7,22 dieno, 4) 3β, 5α, 6β, 9α-tetrahydroxyergosta-7, 22-dien and 5) 3β, 5α, 9α trihidroxiergosta-7,22-dien-6-one.

    Keyworks: Mushrooms, functional foods, active secondary metabolites.
  • beenhereReferences
    • Alarc.n, J., .guila, S., Arancibia-.vila, P., Fuentes, O., Zamorano- Ponce, E., & Hern.ndez, M. (2003). Production and purification of statin from Pleurotus ostreatus (basidiomycetes) strains. Zeitschrift für Naturforschung C, 58, 62–64.

    • Bok, J. W., Lermer, L., Chilton, J., Klingeman, H. G., & Towers, G. H. N. (1999). Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry, 51, 891–898.

    • Chegwin-Angarita, C., Nieto-Ram.rez, I., Atehort.a, L., & Sep.lveda, A. L. (2012). Las estatinas: Actividad biol.gica y producci.n biotecnol.gica. Revista Colombiana de Biotecnología, 14(2), 157–178.

    • Cohen, R., Persky, L., & Hadar, Y. (2002). Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Applied Microbiology Biotechnology, 58, 582–594.

    • Eriksson, K. E. L., Blanchette, R. A., & Ander, P. (1990). Microbial and enzymatic degradation of wood and wood components. Berlin, Germany: Springer- Verlag

    • Ghorai, S., Prosad, B. S., Verma, D., Chowdhury, S., Mukherjee, S., & Khowala, S. (2009). Fungal biotechnology in food and feed processing. Food Research International, 42, 577–587.

    • Kawagishi, H., Katsumi, R., Sazawa, T., Mizuno, T., Hagiwara, T., & Nakamura, T., (1988). Cytotoxic steroids from the mushroom Agaricus blazei. Phytochemistry, 27(9), 2777– 2779.

    • Kobori, M., Yoshida, M., Ohnishi-Kameyama, M., & Shinmoto, H. (2007). Ergosterol peroxide from an edible mushroom suppresses inflammatory responses in RAW264.7 macrophages and growth of HT29 colon adrenocarcinoma cells. British Journal of Pharmacology, 150, 209–219.

    • Mizushina, Y., Takahashi, N., Hanashima, L., Koshino, H., Esumi, Y., Uzawa, J., … Sakaguchi, K. (1999). Lucidenic acid O and lactone, new terpene inhibitors of eukaryotic DNA polymerases from a basidiomycete Ganoderma lucidum. Bioorganic and Medicinal Chemistry, 7(9), 2047–2052.

    • Mora, V. M., & Mart.nez-Carrera, D. (2007). Investigaciones básicas, aplicadas y socioecon.micas sobre el cultivo de setas (Pleurotus) en M.xico. En E. S.nchez, D. Mart.nez- Carrera, G. Mata, & H. Leal (Eds.), El cultivo de setas Pleurotus spp. en México (pp. 69–110).

    • Ramos-Ligonio, A., L.pez-Monteon, A., & Trigos., .. (2012). Trypanocidal activity of ergosterol peroxide from Pleurotus ostreatus. Phytotherapy Research, 26(6), 938–943.

    • Subbiah, M. T. R., & Abplanalp, W. (2003). Ergosterol (major sterol of baker’s and brewer’s yeast extracts) inhibits the growth of human breast cancer cells in vitro and the potential role of its oxidation products. International Journal for Vitamin and Nutrition Research, 73, 19–23.

    • Takaku, T., Kimura, Y., & Okuda, H. (2001). Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. Journal of Nutrition. 131, 1409–1413.

    • Trigos, .., Amezcua, C., Reyna, S., & Carri.n, G. (1997). Cerevisterol from cultures of Verticilium lecani. Micologia Neotropical Aplicada, 10, 56–62.

    • Trigos, .., & Ortega-Regules, A. (2002). Selective destruction of microscopic fungi through photo-oxidation of ergosterol. Mycologia, 94(4), 563–568.

    • Trigos, .., & Su.rez, J. (2010). Los hongos como alimentos funcionales y complementos alimenticios. En D. Mart.nez- Carrera, N. Curvetto, M. Sobal, P. Morales, & V. M. Mora (Eds.), Hacia un desarrollo sostenible del sistema d e producción-consumo de los hongos comestibles y medicinales en Latinoamérica: Avances y perspectivas en el siglo XXI (pp. 59–76). Puebla, M.xico: Red Latinoamericana de Hongos Comestibles y Medicinales-COLPOS-UNS-CONACYTAMC- UAEM-UPAEP-IMINAP.

    • Trigos, .., Zayas, T., Ortu.o, L., Sobal, M., & Morales, P. (1994). Contenido de ergosterol en algunas especies cultivadas de Pleurotus. Micologia Neotropical Aplicada, 7, 43–46.

    • Tuor, U., Winterhalter, K., & Fiechter, A. (1995). Enzymes of whiterot fungi involved in lignin degradation and ecological determinants for wood decay. Journal of Biotechnology, 41, 1–17.

    • Wasser, S. P. (2011). Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied microbiology and biotechnology, 89,1323–1332.

    • Wiseman, H. (1993). Vitamin D is a membrane antioxidant: Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Letters 326, 285–288.

    • Yazawa, Y., Yokota, M., & Sugiyama, K. (2000). Antitumor promoting effect of an active component of Polyporus, ergosterol and related compounds on rat urinary bladder carcinogenesis in a short-term test with concanavalin A. Biology & Pharmacology Bulletin, 23, 1298–1302.

  • starCite article

    Márquez-Fernández, O., Juárez Pacheco, L. Á.,  &  Trigos, Á. (2014).  ISOLATION AND IDENTIFICATION OF STEROLS IN A COMMERCIAL STRAIN OF Pleurotus sp.. Revista Chapingo Serie Ciencias Forestales y del Ambiente, XX(2), 227-235.