Caracterización molecular mediante iniciadores ISSR de Magnolia mexicana DC. de dos regiones de Zongolica, Veracruz, México
Molecular characterization using ISSR primers of Magnolia mexicana DC. from two regions in Zongolica, Veracruz, Mexico
Available online: 2017-08-02 / pages.427-436
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Introduction: Magnolia mexicana DC. is a threatened species according to NOM-059-SEMARNAT-2010, a situation attributed to the fragmentation and destruction of its habitat. There are no studies on the genetic diversity of M. mexicana, even though it is endemic to our country.Keyworks: Threatened species, fragmented habitat, genetic variability, Shannon-Weaver index.
Objective: To evaluate genetic variability in two M. mexicana populations using ISSR molecular markers.
Materials and methods: The collections come from Amatitla and Zapotla in Zongolica, Veracruz. The DNA was extracted from the young leaves. A total of 55 ISSR primers were tested; the 10 that produced the highest number of polymorphic bands were selected and amplified by PCR.
Results and discussion: The ISSR primers showed 86 % polymorphism. Cluster analysis, using Ward's minimum variance method, was able to separate the collections by their geographical origin. Analysis of molecular variance showed that the greatest variability (90.88 %) is found within each population. The Shannon-Weaver diversity index was 0.47 and 0.41 for Amatitla and Zapotla, respectively.
Conclusion: The M. mexicana populations have not undergone changes in their genetic structure; there is no evidence at genetic level of alterations caused by population reduction or habitat fragmentation.
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Medrano-Hernández1, J. M., Rodríguez de la O, J. L., Reyes-Trejo, B., & Peña-Ortega, M. G. (2017). Molecular characterization using ISSR primers of Magnolia mexicana DC. from two regions in Zongolica, Veracruz, Mexico. Revista Chapingo Serie Ciencias Forestales y del Ambiente, XXIII(3), 427-436. http://dx.doi.org/10.5154/r.rchscfa.2017.03.019