Revista Chapingo Serie Ciencias Forestales y del Ambiente
Universidad Autónoma Chapingo
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Revista Chapingo Serie Ciencias Forestales y del Ambiente
Volume XXV, issue 2, May - August 2019
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Actividad proteolítica de frutos silvestres de Bromelia karatas L. de Yucatán, México
Proteolytic activity of wild fruits of Bromelia karatas L. of Yucatán, Mexico

Hernán de J. Villanueva-Alonzo; Glendy M. Polanco-Hernández; Gabriel Lizama-Uc; Karla Y. Acosta-Viana; Arturo A. Alvarado-Segura

http://dx.doi.org/10.5154/r.rchscfa.2018.08.062

Received: 2018-08-16

Accepted: 2019-01-25

Available online: 2019-04-02 / pages.157-168

 

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  • descriptionAbstract

    Introduction: Bromeliads are a source of proteases that have not been fully studied.
    Objectives: To evaluate the proteolytic activity of wild fruits of Bromelia karatas L. under different temperature, pH and NaCl conditions, and to estimate the thermal stability of their proteases.
    Materials and methods: The effect of pH (6, 7, 8, 9, 10 and 12), incubation temperatures (30, 40, 50, 60 and 70 °C) and NaCl concentrations (5, 10 and 20 %) on the proteolytic activity of the fruits was compared. The thermal stability of the proteases was assessed at 30, 50 and 70 °C for 240 minutes. The molecular weights and isoelectric points of the proteases were estimated by non-reducing two-dimensional zymography. The data were analyzed using a one-factor ANOVA and Tukey's test (α = 0.05).
    Results and discussion: The proteolytic activity of B. karatas fruit extract (8.59 U·mg-1) was greater than that of Ananas comosus [L.] Merr. variety comosus (3.42 U·mg-1). The activity was higher at pH 6 and 7 and in concentrations lower than 5 % NaCl. Proteases were stable at 30 and 50 °C for 210 minutes. The two-dimensional zymogram, under non-reducing conditions, showed at least 40 light zones with apparent molecular weights between 27.3 and 290 kDa, potentially representing proteases.
    Conclusion: The proteases of B. karatas have potential for application in the food industry.

    Keyworks: Bromeliaceae; proteases; karatasin; 2D zymography
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  • starCite article

    Villanueva-Alonzo, H. d.,  Polanco-Hernández, G. M.,  Lizama-Uc, G., Acosta-Viana, K. Y.,  &  Alvarado-Segura, A. A. (2019).  Proteolytic activity of wild fruits of Bromelia karatas L. of Yucatán, Mexico. Revista Chapingo Serie Ciencias Forestales y del Ambiente, XXV(2), 157-168. http://dx.doi.org/10.5154/r.rchscfa.2018.08.062