PROPAGACIÓN CLONAL IN VITRO DE Eucalyptus pellita, F. MUELL.
In vitro CLONAL PROPAGATION OF Eucalyptus pellita F. MUELL
Available online: / pages.29-33
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Propagation of superior genotypes of Eucalyptus pellita is a promising alternative for afforestation and breeding programs. This paper describes the technology for the clonal propagation of selected trees of E. pellita to obtain seedlings of high quality for planting directly in the field or for establishing seed orchards. Young shoots from selected trees were cut as uninodal stumps and disinfected in a sodium hypochloride solution. Survival of aseptically established node segments was 38.3 %. The segments were transferred to culture media containing auxins and cytokinins. Eight treatments were designed to study the effect of the hormones and their interaction. An average of 25.4 small stems per cultured bud was obtained every 30 days during the first three subcultures. The multiplication rate increased with the number of subcultures, and large masses of buds were observed. For root induction, the liquid media tested were supplemented with IBA and IAA. The treatment in which both auxins were combined at 2 mg⋅L-1 provided 85 % rooted explants with a good connection between the vascular system of the stem and the root. The acclimatization step developed under controlled conditions in a nursery with 80 % survival at 20 days.Keyworks: Biotechnology, Micropropagation Eucalyptus, Eucalyptus pellita F. Muell.
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Noda-Jiménez, A. L., Álvarez-Olivera, P. A., Junco-Cruz, L., García-López, M., & Sotolongo-Sospedra, R. (2000). In vitro CLONAL PROPAGATION OF Eucalyptus pellita F. MUELL. , VI(1), 29-33. http://dx.doi.org/