Revista Chapingo Serie Ciencias Forestales y del Ambiente
Universidad Autónoma Chapingo
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Revista Chapingo Serie Ciencias Forestales y del Ambiente
Volume VII, issue 2, July - December 2001
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EMBRIOGENESIS SOMÁTICA A PARTIR DE CALLOS DE NIM (Azadirachta indica A. Juss)
SOMATIC EMBRYOGENESIS FROM NIM (Azadirachta indica A. Juss) CALLUS CULTURES

R. Martínez-Ruiz; José Luis Rodríguez-de la O; J. Jesús Vargas-Hernández; H. S. Azpiroz-Rivero

http://dx.doi.org/1111

Received: 2002-01-29

Accepted: 2002-02-11

Available online: / pages.107-114

 

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  • descriptionAbstract

    With the purpose to induce directly the creation of somatic embryos on calls, it was used explants of Neem in MS culture media (Murashige and Skoog, 1962) at different concentrations of benzyl adenine and indol acid. After that the calls were sub cultured in media with minerals of MS in different concentrations of GA3, malta extract, BA and AIA. It was possible to differentiation in globular structures or proembryos usig a microscopic stereoscopic with plan lens 1x and 10x21. The quantification of calls frequency was though the percent of observable value. The embryos structure development and germination of embryos were obtained combining AG3(1.0 mg·l-1) plus Benzyl adenine (0.3 mg·l-1) and Malt extract (500 mg·l-1).

    Keyworks: neem, Azadirachta indica A. Juss, embryogenesis, in vitro propagation.
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  • starCite article

    Martínez-Ruiz, R., Rodríguez-de la O, J. L.,  Vargas-Hernández, J. J.,  &  Azpiroz-Rivero, H. S. (2001).  SOMATIC EMBRYOGENESIS FROM NIM (Azadirachta indica A. Juss) CALLUS CULTURES. Revista Chapingo Serie Ciencias Forestales y del Ambiente, VII(2), 107-114. http://dx.doi.org/1111