Revista Chapingo Serie Horticultura
Universidad Autónoma Chapingo
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Vol.5, núm.2 - 1999
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EFFECT OF N-NO3:N-NH4 RATIO ON NITRATE REDUCTASE AND GLUTAMINE SYNTHETASE ACTIVITY IN Opuntia ficus-indica (L.) Mill.
EFECTO DE LA RELACIÓN N-NO3:N-NH4 SOBRE LA ACTIVIDAD DE LA NITRATO REDUCTASA Y GLUTAMINO SINTETASA EN Opuntia ficus-indica (L.) Mill.

Clemente Gallegos-Vázquez; R. Vázquez-Alvarado; E. Olivares-Sáenz; F. Zavala-García; M. Ortega-Escobar

http://dx.doi.org/10.5154/r.rchsh.1998.10.069

Recibido: 1998-10-22

Aceptado: 1999-04-12

Fecha de publicación en línea: 2016-07-01 /pág.109-114

 

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  • descriptionAbstract

    The objectives of this research were: a) to study the effect of several N-NO3:N-NH4 ratios on the activity of the enzymes nitrate reductase (NR) and glutamine synthetase (GS) and b) to identify the NO3- assimilation sites in prickly pear cactus (Opuntia ficus-indica (L.) Mill.). Five N-NO3:N-NH4 ratios (mg⋅liter-1 N-NO3:mg⋅liter-1 N-NH4) were studied [1:0 (150:0), 2:1 (100:50), 1:1 (75:75), 1:2 (50:100, and 0:1 (0:150)]. An experimental design of random complete blocks was used with four replications with a factorial ar-rangement of treatments. In shoot (cladode) and root, the activity of the enzymes nitrate reductase (NR) and glutamine synthetase (GS) were evaluated in vivo at 21 days after establishment of the experiment. High NR and GS enzyme activity was registered when the nutrient solution was supplied with a higher ratio of N-NO3:N-NH4, thus confirming the dependence of NR activity on the concentration of N-NO3 in the nutrient solution. Both enzymes had similar behavior, and more activity was detected in the roots.

    Keyworks: Prickly pear, nitrogen form, enzymes, hydroponics, nutrition.

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  • starCite article

    Gallegos-Vázquez, C., Vázquez-Alvarado, R., Olivares-Sáenz, E., Zavala-García, F., &  Ortega-Escobar, M. (1999).  EFFECT OF N-NO3:N-NH4 RATIO ON NITRATE REDUCTASE AND GLUTAMINE SYNTHETASE ACTIVITY IN Opuntia ficus-indica (L.) Mill.. Revista Chapingo Serie Horticultura, 5(2), 109-114. http://dx.doi.org/10.5154/r.rchsh.1998.10.069

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